RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics.
•Tumors “educate” platelets (TEPs) by altering the platelet RNA profile •TEPs provide a RNA biosource for pan-cancer, multiclass, and companion diagnostics •TEP-based liquid biopsies may guide clinical diagnostics and therapy selection •A total of 100–500 pg of total platelet RNA is sufficient for TEP-based diagnostics
Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy....